Method for deodorization of excrements

ABSTRACT

This invention is a method for deodorization of excrements which is characterized by cultivating a microorganism of Lactobacillus, said microorganism having the ability to deodorize or reduce the odor of raw excrements or a diluent thereof when cultivated with these materials, and then administering to human beings or animals the living cells of this microorganism as such or in admixture with a feed.

This invention relates to a method for deodorization of human beings' or animals' excrements or night soil, and especially to a harmless method of propagating a microorganism in the body of the said animals thereby enabling them to discharge excrements with no or remarkably reduced odor for a long time. More particularly, it relates to isolating and collecting a microorganism of the genus Lactobacillus which has the ability to deodorize or reduce the odor of excrements or a diluent thereof when cultivated with these materials, producing a large amount of living cells thereof by cultivation, and then administering to human beings or animals the said living cells as such as in admixture with various substances.

In the following, human beings and animals are all simply referred to as animals.

While investigating microorganisms belonging to Lactobacillus, we have found strain No. 1946/F.R.I. and a new group of Lactobacillus strains which have the ability of deodorize raw excrements or a diluent thereof when cultivated with these materials. Simultaneously, we confirmed that, when the living cells obtained by cultivating these microorganisms or a mixture thereof with a feed administered to animals, excrements discharged from said animals are free of its peculiar odor. We also confirmed that all the microorganisms such as, Strain No. 1946/F.R.I. isolated by us, and belonging to the genus Lactobacillus, remain stable in the animals' intestines, utilize or change the substances giving out the peculiar odor of excrements and are non-pathogenic. Further, from the results obtained it was presumed that the microorganisms belonging to Lactobacillus are not only non-pathogenic but also serve to bind the bowels.

The definition of the genus Lactobacillus which has been recognized heretofore is shown as follows:

It is gram-negative, usually anaerobic and forms no spores. It's shape may be coccus-like, bended, corynelike or thread-like, but not ramified significantly. It is non-motile, catalase negative and does not reduce nitrates. It does neither decompose gelatin nor produce indole and hydrogen sulfide. Some strains are bipolarstained. The ability to decompose protein and fat is very weak, if any. It shows better growth under anaerobic or microaerophilic conditions rather than under aerobic conditions. It has potent ability to decompose sugar. It is acid-fast, and glucose-fermentation of the strains gives lactic acid in an yield of more than 50%. It is not pathogenic to both animals and plants.

Known Lactobacillus can grow only in a medium containing relatively good nutrients. Scientifically, this has been well-recognized as the nutritive requirements of Lactobacillus. Similarly, in the academic circle and in research there has been known no Lactobacillus microorganism which can squeeze into the established cluster of intestinal microbes or thrust aside other microbes thereby enabling itself to propagate and remain stable in the intestines for a lone time. As a matter of course, such Lactobacillus has not been isolated, collected and/or cultivated up to now.

Unlike those mentioned above, however, it has been found that Strain No. 1946/F.R.I. and other microorganisms isolated by the inventors can grow and propagate very well within a short time (e.g., 2 days) even under poor nutritional conditions, produce lactic acid, have the ability of deodorization, and further remain stable in the intestines. It has also been found that, when these microorganisms are administered to animals, the excrements discharged does not show it's peculiar odor and said loss of odor continues for a long time. Further, in addition to the microorganisms isolated by the inventors as having the ability to deodorize excrements for a long time, there were also isolated microorganisms which have the ability to deodorize excrements for (a) a fairly long time (about half a month), (b) a short time (about 5 to 10 days), (c) a considerably short time (about 2 to 3 days) as well as (d) microorganisms having the ability to reduce the odor of excrements for 2 to 7 days. The above-mentioned microorganisms of group (a) isolated by the inventors include Strain No. 2781/F.R.I. On the other hand, the microorganisms of group (b), (c) and (d) include Strain No. 2782/F.R.I., Strain No. 2779/F.R.I. and Strain No. 2780/F.R.I., respectively.

A rather high interrelation was recognized between the deodorizing ability and nutritive requirements of these microorganisms. Examples of said interrelation are shown in Tables 8 and 9.

All the properties of the Strain No. 1946/F.R.I. isolated by the inventors were identical with the aforementioned properties of Lactobacillus. Additionally, almost all of the microorganisms which showed the ability to deodorize raw excrements or a diluent thereof when cultivated therewith were identified to belong to the genus Lactobacillus. Moreover, as already mentioned in defining Lactobacillus, the shape of these microorganisms may vary such as rod-like, typical, oval rod-like or coccus-like. Further, it was recognized that some of these microorganisms change their shape to a remarkable extent by the difference of nutrients. For example, even the same microorganism changed its shape from rod-like to oval rod-like or coccus-like according to the medium employed. Accordingly, the microorganisms collected could not be classified into one or two species but covered a wide variety of Lactobacillus. Of those, the microorganisms belonging to L. salivarius, L. acidophilus and L. delbruckii were observed most frequently. On the other hand, 50% of the microorganisms could not be classified to any one of the known species. Besides, many strains having the productivity of antibiotics were also found among the isolated microorganisms.

Amino acids, peptides, nucleic acid analogs, vitamins, salts, fatty acids or their esters, sugars and so forth are required for the growth of Lactobacillus known heretofore. On the contrary, as will be seen in Tables 1 to 2, Lactobacillus isolated and collected by the inventors and which have the ability of deodorization need only remarkably poor nutrients. From this fact it is considered that the microorganisms isolated are a new group of Lactobacillus.

As for the biochemical properties of Lactobacillus employed in the present invention, those of the strain Strain No. 1946/F.R.I. are shown in Table 3 representatively.

Further, the living cells of the microorganism picked up by a loop or a culture broth of the microorganism were cultivated with four samples of animals' excrements, i.e., fresh and old excrements and their 5-fold diluents. The result of the experiments as to the decrease of odor of excrements are shown in Tables 1 to 5.

In this invention, the Strain No. 1946/F.R.I. and the like is the deposit number of the strains of this invention accepted by the Fermentation Research Institute (Agency of Industrial Science and Technology) of Japan.

                                      TABLE 1                                      __________________________________________________________________________     Relation between nutritive requirement and growth                                        Compounds added to the basic medium                                        Basic    Sulfur-                                                               medium   contain-                                                                            Cyclic                                                                             Branched                Casamino                                                                            Casamino                  Micro-    No   ing  amino                                                                              amino                                                                               Cystein                                                                            Cystine                                                                             Methio-                                                                             Casamino                                                                            acid +                                                                              acid                                                                                Yeast                organism  addition                                                                            amino                                                                               acid                                                                               acid          nine acid vitamin                                                                             yeast                                                                               extract                             acid                                  extract                   __________________________________________________________________________     Strain No.                                                                           (A) --   +++  ⊥                                                                             +    ++  ++   +    ++   ++   ++   ++                   1946/F.R.I.                                                                          (B) --   +    +   -    +   +    +    +    ++   ++   ++                   __________________________________________________________________________            (A):                                                                              S.W medium + agar    S.W. medium components:                                                                       KH.sub.2 PO.sub.4                                                                     1 g                              (B):                                                                              S.W medium (agar was not added)     MgSO.sub.4.7H.sub.2                                                                   0.7 g                            +: Growth in normal condition          NaCl   1 g                              ++:                                                                               Growth in the better condition      (NH.sub.4).sub.2 HPO.sub.4                                                            4 g                              +++:                                                                              Growth in the best condition        FeSO.sub.4.7H.sub.2                                                                   0.03 g                           ⊥:                                                                           Growth in the growing condition slightly                                                                           Glucose                                                                               5 g                              -: No growth                                                            __________________________________________________________________________

                                      TABLE 2                                      __________________________________________________________________________     Relation between nutritive requirement and growth                              Micro- Basic                                                                              Compounds added to the basic medium                                 organism                                                                              medium                                                                             No   Acetic                                                                             Ammonia                                                                             Propionic                                                                            Na.sub.2 S                                                                         Butyric                                                                             Skadol                                                                             Excrements                                    addition                                                                            acid     acid      acid     juice                              __________________________________________________________________________            A    -   -   -    -     -   -    -   +                                  Strain No.                                                                            B   -    -   -    -     ++  -    +   +                                  1946/                                                                          F.R.I.                                                                                C   +    +   -    -     ++  ++   +   + +                                       D   +    ++  -    -     ++  ++   +   ++                                 __________________________________________________________________________               (A): S.W medium (No agar)                                                                       Components of S.W medium:                                     (B): S.W medium + agar                                                                          They are identical with those                                 (C): Peptone 8 g disclosed in Table 1.                                              Glucose 2 g                                                               (D): Peptone 10 g                                                                   Meat extract 5 g                                                               NaCl 5 g                                                                       Glucose 1 g                                                     __________________________________________________________________________

                  TABLE 3                                                          ______________________________________                                                 Microorganism                                                          Properties           Strain No. 1946/F.R.I.                                    Microscopic                  +                                                 observation                                                                              Shape          Short rod, non-spore-                                                          forming                                               ______________________________________                                                 Condition for                                                                               Usually anaerobic                                                 cultivation                                                            ______________________________________                                                                  It forms opaque white                                                          and moistened                                         Observation              colinies on a glucose-                                on medium Shape of colony                                                                               agar medium with                                                               vitamin. The colony                                                            is of medium size.                                                             The surface of the                                                             colony is smooth and                                                           shows normal upheaval.                                ______________________________________                                                   Catalase       -                                                               Nitrate reduction                                                                             -                                                               Gelatin liquefaction                                                                          -                                                               Indole         -                                                     Biochemical                                                                              Hydrogen sulfide                                                                              -                                                     properties                                                                               Ammonia production                                                                            -                                                               Urea decomposition                                                                            -                                                               Milk coagulation                                                                              -                                                               Motility       -                                                               Hemolysis      -                                                               Pigment production                                                                            -                                                               Pathogenic property                                                                           -                                                               Antibiotic     +                                                               productivity                                                         ______________________________________                                    

                  TABLE 4                                                          ______________________________________                                                    Microorganism                                                                            Strain No. 1946/F.R.I.                                    ______________________________________                                                      Ribose      -                                                                  Galactose   +                                                                  Sucrose     +                                                                  Maltose     +                                                     Sugar decomposition                                                                         Cellobiose  +                                                                  Lactose     +                                                                  Meliboise   +                                                                  Raffinose   -                                                                  Melecitose  -                                                                  Mannit      -                                                                  Gas production                                                                             -                                                     ______________________________________                                    

                                      TABLE 5                                      __________________________________________________________________________     Experiments as to deodorization of animal's excrements                         by Strain No. 1946/F.R.I. (Fresh excrements were employed)                                  (Control)                                                                             State of microorganisms added to excrements                     Fresh   No addition                                                                           Microbial cells                                                                             Culture broth cultivated                      Animals                                                                             excrements                                                                             of micro-                                                                             (Clots of microbes)                                                                         for 48 hours                                  employed                                                                            (2 g in/total)                                                                         organisms                                                                             3 loopful    (5 c.c)                                       (%)                 Period of cultivation                                                                       Period of cultivation                                             after inoculation (hr.)                                                                     after inoculation (hr.)                                           24  48   72  24  48   72                                   __________________________________________________________________________     Dogs 100     4      2   2     1' 2   2    1'                                        20      4       2' 2     1' 2    1'  1'                                   Cows 100     4       1'  1'   1' 1    1'   1                                        20      4       1' 1    1   2   2    1'                                   Pigs 100     4      2   2     1'  2' 2    1'                                        20      4      2   2     1'  2' 2    1'                                   Chickens                                                                            100     4      2   2     1'  2' 2    1'                                        20      4      2   2     1'  2' 2    1'                                   Human                                                                               100     4       2' 2     1'  2' 2    1'                                   beings                                                                              20      4       2' 2     1' 2   2    1'                                   __________________________________________________________________________

                                      TABLE 6                                      __________________________________________________________________________     Experiments as to deodorization of animal's excrements                         by Strain No. 1946/F.R.I. (Old excrements were employed)                                      (Control)                                                                             State of microorganisms added to excrements                     Old            Microbial cells                                                                             Culture broth cultivated                    Animals                                                                               excrements                                                                             No addition                                                                           (Clots of microbes)                                                                         for 48 hours                                employed                                                                              (2 g in/total)                                                                         of micro-                                                                             3 loopful    (5 c.c.)                                           (%)     organism                                                                              Period of cultivation                                                                       Period of cultivation                                             after inoculation (hr.)                                                                     after inoculation (hr.)                                           24   48 72   24   48 72                                  __________________________________________________________________________     Dogs   100     4      2    2  1     2'  2   1'                                        20      4      2    2  1     2'  2   1'                                 Cows   100     4      2     1'                                                                               1    2     1'                                                                               1                                          20      4      2     1'                                                                               1    2     1'                                                                               1                                   Pigs   100     4      2    2   1'   2'  2   1'                                        20      4      2     1'                                                                                1'   2'  2   1'                                 Chickens                                                                              100     4      2    2   1'   2'  2  2                                          20      4      2    2   1'   2'  2   1'                                 Human beings                                                                          100     4      2    2   1'   2'  2   1'                                        20      5      2    2   1'  2    2   1'                                 __________________________________________________________________________

    In Tables 5 to 6:                                                              Excrements (%):                                                                              100% Excrements 100%                                                           20% Excrements 20% Water 80%                                     Microbial cells:                                                                             3 loopful of the microorganism grown                                           on a Petri dish were inoculated into                                           the above-mentioned excrements.                                  Culture broth:                                                                               5 c.c of a broth of the microorganism                                          grown well in a test tube were                                                 inoculated into the above-mentioned                                            excrements.                                                      Fresh excrements:                                                                            Excrements just dishcarged.                                      Old excrements:                                                                              Excrements elapsed 3 days after                                                discharged.                                                      1:   No odor                                                                   1':  Dim odor                                                                  2:   Little odor is smelt initially, but will fade away                             sooner.                                                                   2':  Little odor                                                               3:   The odor is less than that of the control group                           3':  The odor is dimly weaker than that of the control                              group.                                                                    4:   The odor of excrements per se in the control group.                  

Whether the Lactobacillus strains can propagate in raw excrements and a diluent thereof is extremely important. In this respect, however, it is clear from Table 7 that the Lactobacillus strains can propagate very well in these materials.

                                      TABLE 7                                      __________________________________________________________________________     Degree of propagation of Strain No. 1946/F.R.I. in                             intestinal tract, fresh excrements, a diluent of                               fresh excrements and so forth                                                  Objects to be                                                                             Number of microorganisms                                                                     Results obtained after inoculation of the             administered                                                                              at the time of inocula-                                                                      strain (per 1 c.c)                                               tion (per 1 c.c)                                                                             24 hours                                                                             48 hours                                                                             72 hours                                                                              96 hours                           __________________________________________________________________________     Intestines of human                                                                       Those cultivated in 300                                             beings (Oral                                                                              ml of a skim-milk medium                                                                           1 × 10.sup.9                                                                   1.5 × 10.sup.9                                                                  2 × 10.sup.9                 administration)                                                                           (administered 2 times)                                              Fresh excrements                                                                          1 × 10.sup.8                                                                           4 × 10.sup.8                                                                   1 × 10.sup.9                                                                   1.5 × 10.sup.9                                                                  2 × 10.sup.9                 A diluent of fresh                                                                        1 × 10.sup.8                                                                           5 × 10.sup.8                                                                   1 × 10.sup.9                                                                    2 × 10.sup.9                                                                   2 × 10.sup.9                 excrements                                                                     Skim-milk medium                                                                          1 × 10.sup.8                                                                           6 × 10.sup.8                                                                   3 × 10.sup.9                                                                    5 × 10.sup.9                                                                   5 × 10.sup.9                 __________________________________________________________________________

    In Table 7:                                                                    Intestines of human beings: 300 ml of a broth of the                           strain cultivated in a skim-milk medium were                                   administered. Number of microbial cells in                                     excrements was counted from the day after said                                 administration.                                                                Raw excrements: The strain was inoculated to fresh                             excrements just discharged, well mixed and                                     cultivated for a period of time. Number of                                     microbial cells was counted.                                                   A diluent of fresh excrements: The strain was                                  inoculated to a 5-fold diluent of excrements and                               cultivated for a period of time. Number of                                     microbial cells was counted.                                                   Skim-milk medium: Components of the medium                                     Skim-milk              60 g                                                    Glucose                10 g                                                    K.sub.2 HPO.sub.4      2 g                                                     Yeast extract          2 g                                                     CaCO.sub.3             3 g                                                     pH                     7.2                                                

The strain Strain No. 1946/F.R.I. having the above-mentioned properties was cultivated in a nutrient medium to collect a large amount of the living cells thereof, and said living cells was then administered orally to animals. The deodorizing effect in this case are shown in Tables 8-1, 2 and -3.

In case of Table 8-1, the strain was cultivated in a nutrient medium containing skim-milk, sugar and CaCO₃. In case of Table 8-2, the strain was cultivated in a medium containing sugar and vitamin but not containing animal milk. In the latter case, the living cells was collected by a centrifuge under cooling, processed to make wet cake, mixed with (a) bread or (b) butter, and then administered orally. In case of Table 8-3, the above-mentioned wet cake was administered in admixture with drinking water.

Likewise, the living cells of the strain dispersed uniformly was mixed with a starch solution and then with dried materials such as dried starch, dried and further mixed with foods. Alternatively, the above-mentioned wet cake was mixed with a skim-milk solution containing lactose and then dried in vacuo, or said wet cake was mixed with foods. Oral administration of these products gave the same results as those obtained in the aforementioned cases.

                  TABLE 8-1                                                        ______________________________________                                         Animals employed                                                                          Deodorizing effect and change thereof                               ______________________________________                                                    The deodorizing effect was observed                                            on the 2nd day after the adminis-                                              tration and continued for about 20                                  Dogs       days. Thereafter, the excrements is                                            started to emit its peculiar odor                                              gradually and resumed its natural                                              state.                                                              ______________________________________                                         Pigs       Same as above.                                                      ______________________________________                                                    The deodorizing effect was observed                                            on the 2nd day after the adminis-                                              tration and continued for about 15                                  Chickens   days. Thereafter, the excrements is                                            started to emit its peculiar odor                                              gradually and resumed its natural                                              state.                                                              ______________________________________                                         Human beings                                                                              Same as dogs' case.                                                 ______________________________________                                         Note: Dogs:        Mean Value of 50 dogs.                                            Pigs:        Mean value of 20 pigs.                                            Chickens:    Mean value of 30 chickens.                                        Human beings:                                                                               Mean value of 15 men and 15 women.                          ______________________________________                                    

                  TABLE 8-2                                                        ______________________________________                                         Animals  Type   Deodorizing effect and change thereof                          ______________________________________                                                          The deodorizing effect was observed on                                        the day after the administration and                                    (a)    continued for about 40 days. There-                                            after, the excrements is started to                                            emit its peculiar odor gradually and                           Dogs            resumed its natural state.                                                     The deodorizing effect was observed on                                         the day after the administration and                                           continued for about 45 to 50 days.                                      (b)    Thereafter, the excrements is started to                                       emit its peculiar odor gradually and                                           resumed its natural state.                                     ______________________________________                                                         The deodorizing effect was observed on                                         the day after the administration and                                    (a)    continued for about 30 days. It -  followed same as the                        dogs case above                                                Pigs            thereafter.                                                                    The deodorizing effect was observed on the                                     day after the administration and con-                                   (b)    tinued for about 40 days. It followed                                          same as the dogs case above thereafter.                        ______________________________________                                                         The deodorizing effect was observed on the                                     2nd day after the administration and con-                               (a)    tinued for about 20 days. It followed                          Chickens        same as the dogs case above thereafter.                                        The deodorizing effect was observed on the                                     2nd day after the administration and con-                               (b)    tinued for about 20 days. It followed                                          same as the dogs case above thereafter.                        ______________________________________                                         Human beings                                                                            (a)    Same as dogs case.                                                      (b)    Same as dogs case.                                             ______________________________________                                    

                  TABLE 8-3                                                        ______________________________________                                         Animals employed                                                                          Deodorizing effect and change thereof                               ______________________________________                                                    The deodorizing effect was observed on                              Dogs       the 3rd day after the administration and                                       continued for about 20 days. There-                                            after, the excrement is started to emit                                        its peculiar odor gradually and resumed                                        its natural state.                                                  ______________________________________                                         Pigs       Same as above.                                                      ______________________________________                                                    The deodorizing effect was observed on - the 3rd day after the                 administration and                                                  Chickens   continued for about 15 days. There-                                            after, the excrement is started to emit                                        its peculiar odor gradually and resumed                                        its natural state.                                                  ______________________________________                                         Human beings                                                                              Same as dogs.                                                       ______________________________________                                    

Further, it was confirmed that the strains of Lactobacillus which have the ability to deodorize fresh excrements or a diluent thereof involves various types of microorganisms. For example, some strains of this genus can deodorize excrements for a longer period of time (e.g., few months) than the strain Strain No. 1946/F.R.I. does. Additionally, the deodorizing effect or the duration of the deodorizing effect of some other strains may be influenced by foods.

                                      TABLE 9                                      __________________________________________________________________________     Relation between nutritive requirement and growth                                         Compounds added to the basic medium                                 Micro- Basic    Sulfur-                     Casa-                                                                              Casa-                                                                              Casamino                   organisms                                                                             medium                                                                             No   containing                                                                           Cyclic                                                                             Branched                                                                            Cy- Cy- Methio-                                                                             mino                                                                               mino                                                                               acid                                                                                Yeast                            addition                                                                            amino amino                                                                              amino                                                                               stein                                                                              stine                                                                              nine acid                                                                               acid +                                                                             yeast                                                                               extract                               acid  acid                                                                               acid                  vitamin                                                                            extract                    __________________________________________________________________________     Strain No.                                                                            A   -    +     -   +    ++  +   +    +   +   +    +                     2782/F.R.I.                                                                           B   -    ⊥                                                                               +   +    ⊥                                                                             ⊥                                                                             ⊥                                                                              +   +   +                          __________________________________________________________________________     Strain No.                                                                            A   -    +     +   +    +   ++  +    +   ++  ++   +                     2781/F.R.I.                                                                           B   -    +     +   +    +   +   ⊥                                                                              +   ++  ++   +                     __________________________________________________________________________     Strain No.                                                                            A   -    +     -   +    -   ⊥                                                                             -    +   +   +    +                     2779/F.R.I.                                                                           B   -    ⊥                                                                               +   +    -   -   ⊥                                                                              ++  +   +    +                     __________________________________________________________________________     Strain No.                                                                            A   -    +     ⊥                                                                             +    -   -   -    ⊥                                                                             ⊥                                                                             +    +                     2780/F.R.I.                                                                           B   ⊥                                                                              ⊥                                                                               ⊥                                                                             -    -   -   ⊥                                                                              +   +   +    +                     __________________________________________________________________________      NOTE:                                                                          The media were cultivated at 37°C for 48 hours.                         A: Basic medium (S.W. medium) + Agar                                           B: Basic medium only. Agar was not added.                                

                                      TABLE 10                                     __________________________________________________________________________     Relation between nutritive requirement and growth                              Micro- Basic                                                                              Compounds added to the basic medium                                 organisms                                                                             medium                                                                         No  Acetic   Propionic                                                         addition                                                                           acid Ammonia                                                                            acid Na.sub.2 S                                                                           Butyric acid                                                                       Skadol                                      __________________________________________________________________________     Strain No.                                                                            A   -    ±                                                                               ± ±  +   ±   +                                    2782/F.R.I.                                                                           B   -    -   -    -     -   ⊥ +                                    __________________________________________________________________________     Strain No.                                                                            A   -    +   +    +     +   +      +                                    2781/F.R.I.                                                                           B   -    ++  ++   +     -   ⊥ +                                    __________________________________________________________________________     Strain No.                                                                            A   -    ±                                                                               -    -     +   ±   -                                    2779/F.R.I.                                                                           B   -    -   -    -     -   ⊥ -                                    __________________________________________________________________________     Strain No.                                                                            A   -    -   -    -     -   -                                           2780/F.R.I.                                                                           B   -    -   -    -     -   -                                           __________________________________________________________________________      Note:                                                                          The media were cultivated at 37°C for 24 hours.                         A: Basic medium (S.W. medium) + Agar                                           B: Basic medium only. Agar was not added.                                

A practical embodiment of the present invention is shown in the following lines.

EXAMPLE

10 g of the strain No. 1946/F.R.I. were inoculated to 18 l of a medium containing vitamin and 60 g/l of skim-milk, or to 18 l of boullion containing vitamin. The medium or boullion was cultivated at 37°C for 3 days, and the living cells of the strain was collected by centrifugation under cooling. 30 g of the living cells were administered to a dog, and the odor change of excrements was examined. As a result it was observed that the dog's excrements was deodorized remarkably for about 40 days from the 2nd day of the administration.

On the other hand, 3 loopful of the strain was inoculated to 2 g of fresh excrements charged into a test tube, and cultivated therein at 37°C for 3 days. In this case, the remarkable deodorizing effect was also observed. 

What is claimed is:
 1. A method for deodorization of excrements of human beings or animals which comprises administering to said human beings or animals the living cells of a microorganism selected from the group consisting of:a. Lactobacillus F.R.I. Strain No. 1946; b. Lactobacillus F.R.I. Strain No. 2779, c. Lactobacillus F.R.I. Strain No. 2780, d. Lactobacillus F.R.I. Strain No. 2781 and e. Lactobacillus F.R.I. Strain No.
 2782. 2. A method for the deodorization of excrements of human beings or animals as claimed in claim 1, wherein said microorganism is Lactobacillus F.R.I. Strain No.
 1946. 3. A method for the deodorization of excrements of human beings or animals, as claimed in claim 1, wherein said microorganism is Lactobacillus F.R.I. Strain No.
 2779. 4. A method for the deodorization of excrements of human beings or animals as claimed in claim 1, wherein said microorganism is Lactobacillus F.R.I. Strain No.
 2780. 5. A method for the deodorization of excrements of human beings or animals as claimed in claim 1 wherein said microorganism is Lactobacillus F.R.I. Strain No.
 2781. 6. A method for the deodorization of excrements of human beings or animals as claimed in claim 1 wherein said microorganism is Lactobacillus F.R.I. Strain No.
 2782. 7. A method according to claim 1 wherein the microorganism is administered to human beings. 